iThe Department of Transportation’s operating agencies have announced their random drug and alcohol testing rates for 2021. The random test rates did not change for 2021.
| Agency | 2021 Random Drug Testing Rate | 2021 Random Alcohol Testing Rate |
| Federal Aviation Administration | 25% | 10% |
| Federal Motor Carrier Administration | 50% | 10% |
| Federal Railway Administration | 25% covered service | 10% covered service |
| Federal Railway Administration | 50% maintenance-of-way | 25% maintenance-of-way |
| Federal Transit Administration | 50% | 10% |
| Pipeline and Hazardous Materials Safety Administration | 50% | N/A |
| United States Coast Guard | 50% | N/A |
