On December 21, 2021, the European Union (EU) Observatory for Nanomaterials (EUON) published a Nanopinion entitled “ACEnano: Goodbye, Hello” by Dr. Eugenia Valsami-Jones about Project ACEnano, a Horizon2020 project that recently completed its 4.5-year course. The project’s main goal was to strengthen clarity into nanomaterial risk assessment using a tiered approach to the physicochemical characterization of nanomaterials. Valsami-Jones describes the analytical solutions produced by the project, some of which are already available to use (e.g., in mass spectrometry, instrument hyphenation, and automated exposure) and others that require further development but have progressed sufficiently to prove their value (e.g., assays and sample introduction system). Valsami-Jones notes that “[a] further ambition of the project was to create a ‘conceptual toolbox’ to hold all of the project’s analytical achievements, some of which were specifically aimed towards demystifying and simplifying analysis, through interlaboratory comparisons, reliable protocol development and making all these accessible via a centralised hub to the community.”